2. Methods

Methods for analysis (Quoted directly and edited from Madsci.org)

We will be using the Turbidity sensor to measure how much algae is in the water.

Alternate methods:
NUMERICAL: cell counting:
This method requires knowledge about taxonomy as this method is used to determine what algae is present in the sample. The sample would be placed under a microscope and the organisms present would be counted. This method is used to determine specifically ow many of a certain organism is present

PROXIMATE: Chlorophyll-a:
The proximate method relies on the usage of a fluorometer or spectrophotometer. This allows one to determine how much algae there is since chlorophyll-a would have a measurable wavelength. In our experiment, we used a variant of the above-mentioned sensors called a turbidity sensor.

GRAVIMETRIC: an almost no-equipment method:

The gravimetric method relies on weighing the dry mass of biomass present in the sample. This would involve filtering the sample after collection and collecting the resulting biomass. The biomass would be left to dry in a low temperature oven and the final weight of the biomass measured.
Figure 1: This is our experiment setup. The control samples are placed at the top while each of the remaining 10 samples of each run are placed at different levels of the shelf.

1 comment:

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