Laboratory logs

ISS Laboratory Logs
Monday(26/1/2015)
-Today Mr Tan returned the first draft of our ISS Proposal. We looked through our proposal and did corrections to the proposal as required. We looked through Mr Tan’s comments and changed to a different scenario where we have fertiliser running off stream instead of our neighbouring fish farmer’s fish dying. We also forgotten to include the our Independent, Dependent and Constant Variables are. We also made major changes to our experimental procedure and safety precautions. We also realised that our bibliography format is done wrongly. We have edited the proposal since then.

Tuesday(27/1/2015)
- We obtained the equipment required for the experiment. We also decided on using the fluorometer for measurement instead as we did not know which wavelength to use on a spectrophotometer for detecting algae.
We went to look for the Laboratory Staff and we managed to get the equipment needed for our experiment. We got:
- 1L Measuring Jug (Quantity:1)
- 2L Measuring Jug (Quantity:1)
-  Measuring Cylinder (Quantity:1)
- Turbidity Sensor (Quantity:1)
- LabQuest 2 DataLogger (Quantity:1)
- Syringe (Quantity:2)
- Glass Rod (Quantity:1)
- Metal Spoon (Quantity:1)
- Weighing Scale (Quantity:1)
- Mortar and Pestle (Quantity:1)
- Dropper (Quantity:1)
- Weighing Boat (Quantity:1)
- Basket (Quantity:1)
- 3L Of Deionised Water Quantity:1)
- 5L of Pond Water (Quantity:1)
We got a Turbidity sensor and Fluorometer. After quite some time playing with the Fluorometer, we decided not to use it as we do not know the best wavelength for the algae to absorb the light. So we moved on to the Turbidity Sensor and surprisingly, it was quite easy to learn how to operate it with the Labquest DataLogger. The lab technician told us that we should hold the small bottle by the black cap so our fingerprints does not affect the result and also be careful when handling glassware.

Tuesday(27/1/2015) - After School Laboratory Log 1
  • We went to the Corona Florist & Nursery to purchase fertiliser for the experiment. When we were there, we found out that there were no fertilizers that are specifically nitrate, phosphorus or potassium based.

Thursday(29/1/2015)
  • Today we went back to school and reported to Mr Tan our problem that there was no fertilizers that are specifically nitrate, phosphorus or potassium based. So we asked him if we could buy it from online. He said that it would be very hard so we decided to adjusted the experiment to using two fertilizer of different mixes of NPK(Nitrate, Phosphorus and Potassium) nutrients.

Monday(2/2/2015)
  • Today we continued our discussion about changing the experimental setup and we were informed by Mr Tan that rats may be a concern as they might chew on our cups for our experiment and ruin it. So we thought about a way to overcome that problem.

Tuesday(3/2/2015)
  • Today when we returned to school, we checked with Mr Tan if there were shelves we could use to place our cups on. He told us that there were shelves that we could use from previous year’s ISS Projects and so we started setting up the shelves needed for our experiment.

Tuesday(3/2/2015) - After School Laboratory Log 2
  • We visited Corona Florist & Nursery once again. So, when we went there, we wanted to buy the fertiliser which is suitable . So we got two kinds of fertiliser. They were all good for growing plants. The first one was Plant Care Products and its NPK (Nitrate, Phosphorus and Potassium) nutrient ratio was 8:8:8 with 2mg of fertiliser. The second one was Quality Compound ‘Leafy” and its NPK(Nitrate, Phosphorus and Potassium) nutrient ratio was 15:15:15. After that we went to the Cold Storage Nearby and bought 2 Packs of 50 plastic cups.

Thursday(5/2/2015)
  • Today when we wanted to use the fertiliser for our project later in the day, we found out that the fertiliser came in the form of beads and we not suitable for both dissolving in both the water and measuring the weight of the fertiliser so we had to spend time grounding it.

Thursday(5/2/2015) - After School Laboratory Log 3
  • Today we spent the day grounding Fertiliser A and we managed to ground about half  of a plastic cup of it with two sets of mortar and pestles.

Monday(9/2/2015)
  • Today we continued grounding Fertiliser A and manage to ground two-thirds of a plastic cup for fertiliser A.


Tuesday(10/2/2015)
  • Today we begun doing a test run and found out that using 50g of fertiliser and adding it directly to the sample would cloud the sample so badly that we would not be able to tell whether the turbidity of the sample was from algae growth or the fertiliser. So we decided to scale down the experiment from 5g to 50g in 5g intervals to 1g to 10g in 1g intervals. We also decided to first dissolve the fertiliser in deionised water and then using a syringe, extract the resulting solution with the dissolved nutrients.

Thursday(12/2/2015)
  • Today during class we prepared the setups that we were going to do for Set-up A by first preparing ten cups that we would use later for all three runs, Run 1, 2 and 3. So we poured in 140ml of deionised water with the respective amount of fertiliser. Then using a glass rod we mix the fertiliser and then left them to settle in a cupboard.

Thursday(12/2/2015) - After School Laboratory Log 4
  • Today we begun with the set-up of the experiment. We began by extracting the water from the cup for Run 2 and 3 before mixing it with the pond water and before we disposed the water mixed with the nutrients, we extract the water one more time before putting it back in and then subsequently mixing it with the pond water.


Monday(16/2/2015)
  • Today we had planned our project for the next two weeks so our plan was that:
  • Today(Monday), we would first ground our Fertiliser B today and after school.
  • Tomorrow(Tuesday) We would do what we did on the previous Thursday(The one before the school ended) and then we would set up the cups of water mixed with fertiliser using the cups of Run 3 Fertiliser B and then after that extract some to Set-up all of Run 1:Fertiliser B and half of Run 2:Fertiliser B with controls. (We would still keep the cups of water mixed with Fertiliser using the cups of Run 3 Fertiliser B)
  • The next next day(Wednesday): We would continue setting up the other half of Run 2:Fertiliser B and all of Run Three using the cups of water mixed with Fertiliser using the cups of Run 3 Fertiliser B.
  • Next Week Monday: We would collect our results for Run 1,2 and w:Fertiliser A
  • Next Week Thursday:We would collect back the  results for all of Run 1:Fertiliser B and half of Run 2:Fertiliser B with controls.
  • Next Week Friday: We would collect back the results for the other half of Run 2:Fertiliser B and all of Run Three.
and we also grounded some Fertiliser B during class time.



Tuesday(17/2/2015)
  • During class time, we prepared the setups that we were going to do for Set-up B by first preparing ten cups that we would use later for all three runs, Run 1, 2 and 3. So we poured in 140ml of deionised water with the respective amount of fertiliser. Then using a glass rod we mix the fertiliser and then left them in the cupboard before going for our recess.

Tuesday(17/2/2015) - After School Laboratory Log 5
  • After lunch we went to the lab and begun the experiment. We started filling up the cups with 10ml of the fertiliser with deionised water and then mixing them with a glass rod before we tested the turbidity of the sample and transporting them to the shelves.

Wednesday(18/2/2015) - After School Laboratory Log 6
  • Today immediately after lunch we went to the lab and begun the experiment. We filled the cups with 10ml of the fertiliser with deionised water and then mixing them with a glass rod before we tested the turbidity of the samples and transporting them to the shelves.

Monday(23/2/2015)
  • Today we decided on how we would test the samples during lesson time. Some updates were made to the report.

Monday(23/2/2015) - After School Laboratory Log 7
  • We immediately begun collecting our results for Run 1, 2 and 3 for Fertiliser A.  The results collected, despite not being consistent with the hypothesis on the influence of fertiliser concentrations on algal growth,still showed a massive increase in algal growth with fertiliser, thus partially aligning with our hypothesis.

Tuesday(24/2/2015)
  • Today we begun working on our blog and poster template and doing the video.
Tuesday(24/2/2015) - After School Laboratory Log 9
  • We begun collecting the results for the Run 1. We tabulated the results and managed to draw graphs for the three runs for Fertiliser A. We noticed how the graphs plateau towards 4 and 6 grams.

Wednesday(25/2/2015)  - After School Laboratory Log 10
  • Today we edited our blog, poster and finalised the video contents.

Thursday(26/2/2015)
  • We continued working on our blog and poster. Updates were made with the changes to the proposal and report.

Thursday(26/2/2015) - After School Laboratory Log 11
  • We found out that the samples of Run 1:Fertiliser B and half of Run 2:Fertiliser B with controls were not showing anything conclusive. Further checks suggest that we may have not put enough fertiliser solution in the samples. Time constraints did not allow us to finish checks on the rest of the samples however.

Friday(27/2/2015) - After School Laboratory Log 12

  • The samples of half of Run 2:Fertiliser B and all of Run Three containing Fertiliser B were not showing anything conclusive. Further checks suggest that we may have not put enough fertiliser solution in the samples. We also managed to finish our logbook, video, poster and blog today for submission.

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